QT-EVE-GM-019
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event DBN9004 | DBN-09004-6 (Soybean)
|
| Genetic target: | DBN-09004-6 insert |
| Description: | Quantitative PCR method for detection of soybean event DBN9004 (EURL GMFF, 2023) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GCCGTATCCGCAATGTGTTANNNNNNNNNNNNNNNNNNNNNTTGTTTACAACTCTGTGACCCNNNNNTGAAAGCACACGT TTATGGAGC |
| Amplicon size: | 89 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | S17A01_F / | GCCGTATCCGCAATGTGTTA | 20 bp | insert |
| Primer reverse | S17A01_R / | GCTCCATAAACGTGTGCTTTCA | 22 bp | 3'-host genome |
| Probe | S17A01_P / | FAM-TTGTTTACAACTCTGTGACCC-MGBNFQ | 21 bp |
