QT-EVE-GM-018
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event GMB151 | BCS-GM151-6 (Soybean)
|
| Genetic target: | GMB151 insert |
| Description: | Quantitative PCR method for detection of soybean event GMB151 (EURL GMFF, 2020) |
Method details
| Specificity: | Event specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TCAAATCAACATGGGTGACTAGAAANNNNCAGTACTGGGCCCTTGTGGCGCTNNATCATAGCTATAAACCTATTCAGCAC AATG |
| Amplicon size: | 84 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | PRIM1040 / | TCAAATCAACATGGGTGACTAGAAA | 25 bp | 5'-host genome |
| Primer reverse | PRIM1041 / | CATTGTGCTGAATAGGTTTATAGCTATGAT | 30 bp | insert |
| Probe | TM1789 / | FAM-CAGTACTGGGCCCTTGTGGCGCT-BHQ1 | 23 bp |
