QT-ELE-00-001
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt176 (176) | SYN-EV176-9 (Maize)
|
| Genetic target: | CaMV P-35S |
| Description: | Quantitative PCR method for detection of CaMV 35S promoter (Feinberg et al., 2005) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CGTCTTCAAAGCAAGTGGATTGNNNNNNNNTCTCCACTGACGTAAGGGATGACGCANAATCCCACTATCCTTCGCAAGA |
| Amplicon size: | 79 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | sF / | CGTCTTCAAAGCAAGTGGATTG | 22 bp | CaMV P-35S |
| Primer reverse | sR / | TCTTGCGAAGGATAGTGGGATT | 22 bp | CaMV P-35S |
| Probe | 35S core / | FAM-TCTCCACTGACGTAAGGGATGACGCA-TAMRA | 26 bp |