QT-CON-00-007
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt176 (176) | SYN-EV176-9 (Maize)
|
| Genetic target: | Cry1A(b) / IVS 9 PEPC |
| Description: | Quantitative PCR method for detection of the junction between a synthetic Cry1A(b) gene and the phospho-enol-pyruvate carboxylase (PEPC) intron N.9 (Shindo et al., 2002) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TGTTCACCAGCAGCAACCAGNNNNNNNNNNNNNCCGACGTGACCGACTACCACATCGANNNNNNNNNNNNNNNNNAGATC TGTTCTGCACAAAGTGGAGT |
| Amplicon size: | 100 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | E176 2-5' / | TGTTCACCAGCAGCAACCAG | 20 bp | Cry1A(b) |
| Primer reverse | E176 2-3' / | ACTCCACTTTGTGCAGAACAGATCT | 25 bp | IVS 9 PEPC |
| Probe | E176-Taq / | FAM-CCGACGTGACCGACTACCACATCGA-TAMRA | 25 bp |
