QT-CON-00-006
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt11 | SYN-BT011-1 (Maize)
|
| Genetic target: | Cry1A(b) / IVS 6 ADH1 |
| Description: | Quantitative PCR method for detection of the junction between the Intron 6 from maize alcohol dehydrogenase 1 gene (ADH1) and the synthetic Cry1A(b) gene (Shindo et al., 2002) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
AAAAGACCACAACAAGCCGCNNNNNNNNNNNNNNCGACCATGGACAACAACCCAAACATCANNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNAGTACTTGGTGGAGAACGCATTG |
| Amplicon size: | 127 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Bt11 3-5' / | AAAAGACCACAACAAGCCGC | 20 bp | IVS 6 ADH1 |
| Primer reverse | Bt11 3-3' / | CAATGCGTTCTCCACCAAGTACT | 23 bp | Cry1A(b) |
| Probe | Bt11-2-Taq / | FAM-CGACCATGGACAACAACCCAAACATCA-TAMRA | 27 bp |
