QT-CON-00-004
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON810 | MON-00810-6 (Maize)
|
| Genetic target: | IVS 1 HSP70 / Cry1A(b) |
| Description: | Quantitative PCR method for detection of the junction between the Intron 1 from the maize HSP70 gene and a synthetic Cry1A(b) gene (Shindo et al., 2002) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GATGCCTTCTCCCTAGTGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATACCAAGCGGCCA TGGACAACAANNCAAACATCAACGAGTGCATCC |
| Amplicon size: | 113 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | M810 2-5' / | GATGCCTTCTCCCTAGTGTTGA | 22 bp | IVS 1 HSP70 |
| Primer reverse | M810 2-3' / | GGATGCACTCGTTGATGTTTG | 21 bp | Cry1A(b) |
| Probe | M810-Taq / | FAM-AGATACCAAGCGGCCATGGACAACAA-TAMRA | 26 bp |
