QT-CON-00-002
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 40-3-2 | MON-04032-6 (Soybean)
|
| Genetic target: | CP4-epsps / CTP |
| Description: | Quantitative PCR method for detection of the junction between the chloroplast transit peptide (CTP) sequence and the CP4-epsps gene (Hird et al., 2003) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Quantitative |
| Assay: | Real-time PCR (Multiplex) |
| Amplicon sequence: |
GGATTTCAGCATCAGTGGCTACAGCCTGCATGCTTCACGGTGCAAGCAGCCGGCCCGCAACCGCCCGCAAATCCTCTGGC CTTTCCGG Genbank ID: AY125353.1 |
| Amplicon size: | 88 bp |
| Multiplex method: |
QT-CON-00-002
+
QT-TAX-GM-004
|
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | RR-F / | GGATTTCAGCATCAGTGGCTACA | 23 bp | CTP |
| Primer reverse | RR-R / | CCGGAAAGGCCAGAGGAT | 18 bp | CP4-epsps |
| Probe | RR soya / | FAM-CCGGCTGCTTGCACCGTGAAG-TAMRA | 21 bp |