GMOMETHODS | EURL GMFF

QL-TAX-OS-003 Species specific method icon

Tested in:

Rice

Genetic target:

GOS9

Description:

Qualitative PCR method for detection of rice root-specific GOS9 gene (EURL GMFF, 2008)

Comments:

Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties.

Specificity:

Species specific

Analysis type:

Qualitative

Assay:

Real-time PCR (Simplex)

Amplicon sequence:

TTAGCCTCCCGCTGCAGAGCGGCAGTGTGGTTGGTTTCTTCGGACGCGCGGGAGCACTTGTGGACTCT
Genbank ID: X51909

Amplicon size:

68 bp

Oligos:

Type	Name(s)	Sequence	Length	Target
Primer forward	org1 /	TTAGCCTCCCGCTGCAGA	18 bp	GOS9
Primer reverse	org2 /	AGAGTCCACAAGTGCTCCCG	20 bp	GOS9
Probe	orgp /	FAM-CGGCAGTGTGGTTGGTTTCTTCGG-Dabcyl	24 bp

European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission. Report on the Verification of the Performance of a Method for the Detection of "Bt 63" Rice Using Real-Time PCR. 2008. | 2008
Grohmann Lutz et al. Detection of genetically modified rice: collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice. European Food Research and Technology. 2009. | 10.1007/s00217-008-0964-1 | 2009
ISO/TS 21569-6:2016 Horizontal methods for molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences. 2016. | 2016
BVL. Detection of genetically modified cry1Ab/Ac and P-ubi-cry DNA sequences in rice products using real-time PCR, No. L 15.06-3. Official Compendium of Analytical Methods according to Section 64 LFGB. 2013. | 2013
Grohmann Lutz et al. Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products. Accreditation and Quality Assurance. 2015. | 10.1007/s00769-015-1108-5 | 2015