QL-TAX-OS-002
|
General information
| Tested in: |
Rice
|
| Genetic target: | pld |
| Description: | Qualitative PCR method for detection of rice Phospholipase D alpha 2 gene (pld) (EURL GMFF, 2006) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TGGTGAGCGTTTTGCAGTCTATGTTGTGCTGCCAATGTGGCCTGAAGGACCTCCTGCTAGTGGATCAG Genbank ID: AB001919 |
| Amplicon size: | 68 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | KVM159 / | TGGTGAGCGTTTTGCAGTCT | 20 bp | pld |
| Primer reverse | KVM160 / | CTGATCCACTAGCAGGAGGTCC | 22 bp | pld |
| Probe | TM013 / | VIC-TGTTGTGCTGCCAATGTGGCCTG-TAMRA | 23 bp |
