QL-TAX-GM-008
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Qualitative PCR method for detection of soybean lectin gene (LE1) (DMIF-GEN, 1998) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex) |
| Amplicon sequence: |
GCCCTCTACTCCACCCCCATCCACATTTGGGACAAAGAAACCGGTAGCGTTGCCAGCTTCGCCGCTTCCTTCAACTTCAC CTTCTATGCCCCTGACACAAAAAGGCTTGCAGATGGGC Genbank ID: K00821 |
| Amplicon size: | 118 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | GM03 / | GCCCTCTACTCCACCCCCATCC | 22 bp | LE1 |
| Primer reverse | GM04 / | GCCCATCTGCAAGCCTTTTTGTG | 23 bp | LE1 |
