QL-TAX-GM-001
|
General information
| Tested in: |
Soybean
|
| Genetic target: | LE1 |
| Description: | Qualitative PCR method for detection of soybean lectin gene (LE1) (Barbau-Piednoir et al., 2014) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
AACCGGTAGCGTTGCCAGCTTCGCCGCTTCCTTCAACTTCACCTTCTATGCCCCTGACACAAAAAGGCTTGCAGATGGGC T Genbank ID: K00821 |
| Amplicon size: | 81 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | SLTM 1 / | AACCGGTAGCGTTGCCAG | 18 bp | LE1 |
| Primer reverse | SLTM 2 / | AGCCCATCTGCAAGCCTTT | 19 bp | LE1 |