GMOMETHODS | EURL GMFF

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QL-TAX-DC-001 Species specific method icon

General information

Tested in:

Carnation

Genetic target:

ANS

Description:

Qualitative PCR method for detection of carnation anthocyanidin synthase gene (ANS) (EURL GMFF, 2007)

Comments:

Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties.

Method details

Specificity:

Species specific

Analysis type:

Qualitative

Assay:

Endpoint PCR (Multiplex)

Amplicon sequence:

CTAGATCGGAGGTCACCATACCCGTGAAGATTTTTCTGTGAGAGGAAAAGAACCCAAGGACGAGGTTCAAATCTACACAT
GGAAAGACGCCACGCTTCGTGAGTTAACTGACCTTGTATGTCGCCATCGCTTAGCGTAGCGCTGAACATCGTTTTCACCC
TGCTCCATCCATCAACCATTTATTGGTCTTATACATGTGTGATTGCGTTGTTCTTACATTTAGGTGAAAGACGTTTCTCC
AGCTGCTAGGAGTCGAGATGCGAAATTGTCGTTTGCGACTGTATACCTTGATAGAAATGGATGCATGCAAGTAAAGAAGG
TATCTTCTAATTCATCTTTCGTAGAGACATAGCGTGAATTTGGACGGGGTCTTTGGTTTGAGAAAGATAACAGCTTTACG
TATTTTTGTAGATGGGTGAAACCTTTTCAAATCCGTATAAGCGTAAAGACGACAACTGGGCTTTAGGGGACACATTCTTT
CAGGTATAATTGATGCGACTAACAATAGTCTCCACTGATCATATTCTACTCTTCTACGTTCGATACTGACTGTTTCTGGT
TATTTGGTAGACAGGAGATTATTTGGACGTAGCAATTCAGTAGCGTAGAGATGTTTCCACACGTGTTATCGTAAAAGAAG
CAAGATAAGCCTAATGCCTAGGGTGGTGGTATGACTTCCGTTGCTTATCGATCGTGCTTGTAAGTAATTTCCGTCTTATC
TTTTCCTGTTATATAAAGTTAATCTTCTCTAGGACTTTCATGAACCTTGTTTGTGTATTTATTTCTCGATCAACATGATA
GAGCTAGTTTTTAAGCAACGTATACTAGTAGTCTATTGGAAGTTAAGACACGGTTCTTAAAAAGGTACGATCCAAGTGAA
GCATGTTAGATATGACACTTTCTTCTAGGGACGACTCTCGTATGCCACCCGACTTTTTCAATTTTTTTTGTGAATGTTAG
ATGTGTGTATATAATGCATCCGAAAGATGTCTCAACGAACAAATGAGCCACCTACTTCGATCACTCGCTATCAATGTTAT
TAATGCCTTGTTGATTTTAATAGTTGATCAATAATAGTAAAATCTATTCAAGGGTATAGTCTCCCGTTCACACTCATCGG
GGTTACACTAGCGAGCTCCATTAATCGGTGCCTTAATCGAGACGCTAAGAACTATACCATGACCTAGTCAGCGCCATGGG
ACTGATGTAGGCCACACAATCTCGATGATCCGAAAACGCTAGAGTTCAAGACCTAGTTCGAGACCATGGTCACGGTTTC
Genbank ID: AB727362.2

Amplicon size:

1279 bp

Multiplex method:

QL-TAX-DC-001 Species specific method icon

+ QL-EVE-DC-002 Event specific method icon

QL-TAX-DC-001 Species specific method icon

+ QL-EVE-DC-001 Event specific method icon

QL-TAX-DC-001 Species specific method icon

+ QL-EVE-DC-003 Event specific method icon

QL-TAX-DC-001 Species specific method icon

+ QL-EVE-DC-004 Event specific method icon

QL-TAX-DC-001 Species specific method icon

+ QL-EVE-DC-005 Event specific method icon

QL-TAX-DC-001 Species specific method icon

+ QL-EVE-DC-006 Event specific method icon

Oligos:

Type	Name(s)	Sequence	Length	Target
Primer forward	ANS.F /	CTAGATCGGAGGTCACCATACC	22 bp	ANS
Primer reverse	ANS.R /	GAAACCGTGACCATGGTCTCG	21 bp	ANS

Other methods validated in combination

QL-EVE-DC-001

European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission. Report on the Testing of a PCR-based Detection Method for Identification of FlorigeneTM Moonlite GM Carnation. 2007. | 2007
European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission. Report on the single-laboratory validation of a PCR-based Detection Method for Identification of FlorigeneTM IFD-25958-3 GM Carnation - Validation Report and Validated Method. Luxembourg (Luxembourg): Publications Office of the European Union. JRC77720. 2012. | 10.2788/76760 | 2012
European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission. Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of FlorigeneTM 26407 GM Carnation - Validation Report and Validated Method. Luxembourg (Luxembourg): Publications Office of the European Union. JRC85668. 2013. | 10.2788/34771 | 2013
European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission. Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of SHD-27531-4 GM Carnation - JRC Validated Methods, Reference Methods and Measurement Reports. JRC100171. 2016. | 2016
European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission. Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of GM-line FLO-40685-2 Carnation - JRC Validated Methods, Reference Methods and Measurement Reports. JRC103658. 2016. | 2016