QL-TAX-CP-001
|
General information
| Tested in: |
Papaya
|
| Genetic target: | CHY |
| Description: | Qualitative PCR method for detection of papaya chymopapain gene (CHY) (Wei et al., 2013) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
ATCTACAATCTTGCTAACCCTAAAATGACGGTGCAAGTAGAGTGAATGACAAGATCTTTTATTTAGAGAGAAAGAAACTT GATTGAAAAGACAAGCTAGTATATAAACCCATGCGGATCCTCCCATTTCCCTTCATCCATTCCCACTCTTGAGAGTTAGC TAGTGTTACAATGGCTACGATGAGTTCAATTTCAAAAATTATTTTCTTGGCAACATGTCTCATTATTCATATGGGTTTGT CTTCTGCTGATTTTTACACCGTGGGTTATTCTCAAGATGACT Genbank ID: AY803756 |
| Amplicon size: | 282 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Chy-1F / | ATCTACAATCTTGCTAACCCTA | 22 bp | CHY |
| Primer reverse | Chy-2R / | AGTCATCTTGAGAATAACCCAC | 22 bp | CHY |