QL-TAX-CMV-001
|
General information
| Tested in: |
CMV
|
| Genetic target: | CaMV - ORF V |
| Description: | Qualitative PCR method for detection of CaMV ORF V (ISO/TS 21569-7, 2022) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
ATYAAGCCCAGYAAAAGCCCNNNCATGGCACCAGCCTTCTTGGTCAACAAYGAAGCCGAGAAGCGRAG |
| Amplicon size: | 68 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | CaMV-ORFV-fd2 / | ATYAAGCCCAGYAAAAGCCC | 20 bp | CaMV - ORF V |
| Primer reverse | CaMV-ORFV-rd2 / | CTYCGCTTCTCGGCTTCRTT | 20 bp | CaMV - ORF V |
| Probe | CaMV-ORFV-P2 / | FAM-CATGGCACCAGCCTTCTTGGTCAAC-BHQ1 | 25 bp |