QL-TAX-BN-004
|
General information
| Tested in: |
Oilseed Rape
|
| Genetic target: | HMGa |
| Description: | Qualitative PCR method for detection of oilseed rape high mobility group protein I/Y gene (HMGa) (Pan et al., 2007) |
| Comments: | Taxon-specific methods may generate amplicons with different sequence or length in different plant varieties. |
Method details
| Specificity: | Species specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
GGTCGTCCTCCTAAGGCGAAAGGACCTTCCTCGGAGGTGGAGACGAAAGTTGCGGCACCGAGTGGCTCCGGGAGGCCACG TGGACGACCGCCGAAGAAGCAGAAGACGGAATCCGAGGCGGTTAAAGCCGATGTTGAACCTGCGGAGGCTCCGGCTGGGG AGCGGAGAGGGCGTGGAAGGCCACCGAAAGCGAAGCCTGCGATGGTGCCTGTTGGTTGC Genbank ID: AF127919 |
| Amplicon size: | 219 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | HMG-1 / | GGTCGTCCTCCTAAGGCGAAAG | 22 bp | HMGa |
| Primer reverse | HMG-2 / | GCAACCAACAGGCACCATC | 19 bp | HMGa |
