QL-EVE-ZM-001
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event MON810 | MON-00810-6 (Maize)
|
| Genetic target: | MON810 insert |
| Description: | Qualitative PCR method for detection of maize event MON810 (ISO 21569:2005) |
Method details
| Specificity: | Event specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
TCGAAGGACGAAGGACTCTAACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGACAGTGGTCC CAAAGATGGA |
| Amplicon size: | 170 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | VW01 / | TCGAAGGACGAAGGACTCTAACG | 23 bp | 5'-host genome |
| Primer reverse | VW03 / | TCCATCTTTGGGACCACTGTCG | 22 bp | CaMV P-35S |
