QL-EVE-OS-001
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event LLRICE601 | BCS-OS003-7 (Rice)
|
| Genetic target: | LLRICE601 insert |
| Description: | Qualitative PCR method for detection of rice event LLRICE601 (EURL GMFF, 2006) |
Method details
| Specificity: | Event specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TCTAGGATCCGAAGCAGATCGTNCCACCTCCCAACAATAAAAGCGCCTGNACACTCCGCGCCCTCC |
| Amplicon size: | 66 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | SHA040 / | TCTAGGATCCGAAGCAGATCGT | 22 bp | insert |
| Primer reverse | SHA041 / | GGAGGGCGCGGAGTGT | 16 bp | 3'-host genome |
| Probe | TM098 / | FAM-CCACCTCCCAACAATAAAAGCGCCTG-TAMRA | 26 bp |
