QL-EVE-EC-002
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 19E | (E. coli)
|
| Genetic target: | 19E insert |
| Description: | Qualitative PCR method for detection of E. coli K-12 event 19E (EURL GMFF, 2015) |
Method details
| Specificity: | Event specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GGTTATCCAGTAATAGCCATCTTCATCNNNNNNNNNNCCGTCGCCGCTGTATTGATTCACTTGAAGTACGAAAAAAACCG GGAGG |
| Amplicon size: | 85 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | LMA for / | GGTTATCCAGTAATAGCCATCTTCATC | 27 bp | 5'-host genome |
| Primer reverse | LMA rev / | CCTCCCGGTTTTTTTCGTACTT | 22 bp | insert |
| Probe | LMA probe / | FAM-CCGTCGCCGCTGTATTGATTCACTTG-TAMRA | 26 bp |