QL-EVE-DC-005
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 27531 | SHD-27531-4 (Carnation)
|
| Genetic target: | 27531 insert |
| Description: | Qualitative PCR method for detection of carnation event SHD-27531-4 (EURL GMFF, 2016) |
Method details
| Specificity: | Event specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Multiplex) |
| Amplicon sequence: |
CGAGTAAATTCAAGCATGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAATGAGTATGATGGTCAATATGG |
| Amplicon size: | 458 bp |
| Multiplex method: |
QL-EVE-DC-005
+
QL-TAX-DC-001
|
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 27531 LB Specific fw / | CGAGTAAATTCAAGCATGCCC | 21 bp | 5'-host genome |
| Primer reverse | carLB-reverse / | CCATATTGACCATCATACTCATTGC | 25 bp | insert |