QL-EVE-DC-003
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 25958 | IFD-25958-3 (Carnation)
|
| Genetic target: | 25958 insert |
| Description: | Qualitative PCR method for detection of carnation event 25958 (EURL GMFF, 2012) |
Method details
| Specificity: | Event specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Multiplex) |
| Amplicon sequence: |
TGGACCCTTGAGGAAACTGGTAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGCATAACGCGGTGTACA AG |
| Amplicon size: | 482 bp |
| Multiplex method: |
QL-EVE-DC-003
+
QL-TAX-DC-001
|
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | RB Forward / | TGGACCCTTGAGGAAACTGGTAGC | 24 bp | insert |
| Primer reverse | IFD-25958-3 Reverse / | CTTGTACACCGCGTTATGCTC | 21 bp | 3'-host genome |