QL-ELE-00-024
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event RT73 (GT73) | MON-00073-7 (Oilseed Rape)
|
| Genetic target: | rbcS terminator |
| Description: | Qualitative PCR method for detection of tE9 terminator (Debode et al., 2016) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
TTTTTATTCGGTTTTCGCTATCGAACTGTGAAATGGAAATGGATGGAGAAGAGTTAATGAATGATATGGTCCTTTTGTTC ATTCTCA Genbank ID: X00806 |
| Amplicon size: | 87 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | T-E9-R / | TTTTTATTCGGTTTTCGCTATCG | 23 bp | T-E9 |
| Primer reverse | T-E9-F / | TGAGAATGAACAAAAGGACCATATCA | 26 bp | T-E9 |
| Probe | T-E9-P / | FAM-TCATTAACTCTTCTCCATCCATTTCCATTTCACAGT-TAMRA | 36 bp |