QL-ELE-00-023
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt rice | (Rice)
|
| Genetic target: | CaMV T-35S pCambia |
| Description: | Qualitative PCR method for detection of T35S pCAMBIA sequences (Rischitor et al., 2016) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
CGGGGGATCTGGATTTTAGTACTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAA TACATACTAAGGGTTTCTTATATGCTCAACACATGAGCGAAACCCTATAGGAACCCT Genbank ID: AF234296.1 |
| Amplicon size: | 137 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | t35S pCAMBIA c-F / | CGGGGGATCTGGATTTTAGTA | 21 bp | CaMV T-35S pCambia |
| Primer reverse | t35S pCAMBIA a-R / | AGGGTTCCTATAGGGTTTCGCTC | 23 bp | CaMV T-35S pCambia |