QL-ELE-00-014
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event LLRICE62 | ACS-OS002-5 (Rice)
GMO event MS8 | ACS-BN005-8 (Oilseed Rape) |
| Genetic target: | bar |
| Description: | Qualitative PCR method for detection of phosphinothricin N-acetyl transferase gene (bar) (Grohmann et al., 2009) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
ACAAGCACGGTCAACTTCCNTACCGAGCCGCAGGAACCNNNNGAGTGGACGGACGACCTC |
| Amplicon size: | 60 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | RapB-F1 / | ACAAGCACGGTCAACTTCC | 19 bp | bar |
| Primer reverse | RapB-R1 / | GAGGTCGTCCGTCCACTC | 18 bp | bar |
| Probe | RapB-S1 / | FAM-TACCGAGCCGCAGGAACC-TAMRA | 18 bp |