QL-ELE-00-010
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event RT73 (GT73) | MON-00073-7 (Oilseed Rape)
|
| Genetic target: | P-FMV |
| Description: | Qualitative PCR method for detection of Figwort Mosaic Virus 35S (FMV) promoter (Pan et al., 2007) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
AAGCCTCAACAAGGTCAGGGTACAGAGTCTCCAAACCATTAGCCAAAAGCTACAGGAGATCAATGAAGAATCTTCAATCA AAGTAAACTACTGTTCCAGCACATGCATCATGGTCAGTAAGTTTCAGAAAAAGACATCCACCGAAGACTTAAAGTTAGTG GGCATCTTTGAAAGTAATCTTGTCAACATCGAGCAG Genbank ID: AR016589 |
| Amplicon size: | 196 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | FMV-1 / | AAGCCTCAACAAGGTCAG | 18 bp | P-FMV |
| Primer reverse | FMV-2 / | CTGCTCGATGTTGACAAG | 18 bp | P-FMV |
