QL-ELE-00-006
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 40-3-2 | MON-04032-6 (Soybean)
|
| Genetic target: | T-nos |
| Description: | Qualitative PCR method for detection of nopaline synthase (nos) terminator (BVL, 1998) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
GAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAAT GCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATA TAGCGCGCAAACTAGGATAA Genbank ID: AY125353.1 |
| Amplicon size: | 180 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | NOS-1 / | GAATCCTGTTGCCGGTCTTG | 20 bp | T-nos |
| Primer reverse | NOS-3 / | TTATCCTAGTTTGCGCGCTA | 20 bp | T-nos |
