QL-CON-00-015
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event LLRICE62 | ACS-OS002-5 (Rice)
GMO event LLRICE601 | BCS-OS003-7 (Rice) |
| Genetic target: | CaMV P-35S / bar |
| Description: | Qualitative PCR method for detection of the junction between the CaMV 35S promoter and the bar gene (EURL GMFF, 2006) |
| Comments: | The fragment size generated by the CaMV P-35S-bar PCR depends on the assembly of the two sequence elements and is varying in the different GM plant events. Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
TATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NCAGAACGACGCCCGGCCGACAT TATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAACGACGCCCGGCCGACAT |
| Amplicon size: | 103-136 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | MDB498 / | TATCCTTCGCAAGACCCTTCC | 21 bp | bar |
| Primer reverse | DPA143 / | ATGTCGGCCGGGCGTCGTTCTG | 22 bp | CaMV P-35S |
| Probe | TM099 / | FAM-TCTATATAAGGAAGTTCATTTCATT-MGBNFQ | 25 bp |
