QL-CON-00-013
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event KeFeng6 | (Rice)
|
| Genetic target: | Cry1Ab/Ac / P-ubi |
| Description: | Qualitative PCR method for detection of the junction between the maize ubiquitin promoter and the modified Cry1Ab/Ac gene (Grohmann et al.,2015) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
ATTTGCTTGGTACTGTTTCTTTTGTCNNNNNNNACCCTGTTGTTTGGTGTTACTTCTGCANNNNNACTCTAGAGGATCCA TGGACAACAA |
| Amplicon size: | 90 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Pubi-F2 / | ATTTGCTTGGTACTGTTTCTTTTGTC | 26 bp | P-ubi |
| Primer reverse | Cry-rr-R / | TTGTTGTCCATGGATCCTCTAGAGT | 25 bp | Cry1Ab/Ac |
| Probe | Pubi-T2 / | FAM-ACCCTGTTGTTTGGTGTTACTTCTGCA-NFQ | 27 bp |
