QL-CON-00-012
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 55-1 | CUH-CP551-8 (Papaya)
GMO event 63-1 | CUH-CP631-7 (Papaya) |
| Genetic target: | CaMV P-35S / CMV/PRSV CP |
| Description: | Qualitative PCR method for detection of the junction between the CaMV 35S promoter and the CMV/PRSV sequence (ISO 21569: 2005/Amd.1:2013) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
TCATTCTTGGACTGACGACGTCGATGGTTACGACCAGCACTGGTTGATTCAGATTTGTCCATGACTCGACTCAATTCTAC GACACAAAAGAGAAAACACAGCACACACACTCTCTATATAGTCAGTAGGGGTACCCTGTCCTCTCCAAATGAAATGAACT TCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTC Genbank ID: FJ467933 |
| Amplicon size: | 220-0 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 35S-F / | GACGTAAGGGATGACGCACAA | 21 bp | CaMV P-35S |
| Primer reverse | sunup-ar1 / | TCATTCTTGGACTGACGACGT | 21 bp | CMV/PRSV CP |
| Probe | 35S -T / | FAM-CCCACTATCCTTCGCAAGACCCTTCC-TAMRA | 26 bp |