QL-CON-00-007
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt63 (TT51-1) | (Rice)
|
| Genetic target: | Cry1Ab/Ac / T-nos |
| Description: | Qualitative PCR method for detection of the junction between Cry1Ab/Ac and DNA spacer sequences (Grohmann and Maede, 2009) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
GACTGCTGGAGTGATTATCGACAGANTCGAGTTCATTCCAGTTACTGCAACACTCGAGNCTGAATAAGTCGAGGTACCGA GCT |
| Amplicon size: | 83 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | T51F / | GACTGCTGGAGTGATTATCGACAGA | 25 bp | Cry1Ab/Ac |
| Primer reverse | T51R / | AGCTCGGTACCTCGACTTATTCAG | 24 bp | DNA spacer sequences |
| Probe | T51p / | FAM-TCGAGTTCATTCCAGTTACTGCAACACTCGAG-TAMRA | 32 bp |
