QL-CON-00-006
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event 40-3-2 | MON-04032-6 (Soybean)
|
| Genetic target: | CaMV P-35S / CTP |
| Description: | Qualitative PCR method for detection of the junction between the CaMV 35S promoter and the chloroplast transit peptide (CTP) sequence (EU-Project SMT4-CT96-2072:1998) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex) |
| Amplicon sequence: |
TGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAG TTCATTTCATTTGGAGAGGACACGCTGACAAGCTGACTCTAGCAGATCTTTCAAGAATGGCACAAATTAACAACATGGCT CAAGGGATACA Genbank ID: AX033493 |
| Amplicon size: | 171 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | p35S-af2 / | TGATGTGATATCTCCACTGACG | 22 bp | CaMV P-35S |
| Primer reverse | petu-ar1 / | TGTATCCCTTGAGCCATGTTGT | 22 bp | CTP |
