QL-CON-00-003
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt11 | SYN-BT011-1 (Maize)
|
| Genetic target: | IVS 2 ADH1 / pat |
| Description: | Qualitative PCR method for detection of the junction between the intron 2 from the maize ADH1 gene and the pat gene (ISO 21569:2005) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex) |
| Amplicon sequence: |
CTGGGAGGCCAAGGTATCTAATCAGCCATCCCATTTGTGATCTTTGTCAGTAGATATGATACAACAACTCGCGGTTGACT TGCGCCTTCTTGGCGGCTTATCTGTCTCAGGGGCAGACTCCCGTGTTCCCTCGGATCTCGACATGTCTCCGGAGAGGAGA CCAGTTGAGATTAGGCCAGCTACAGCAGC Genbank ID: AR110602 |
| Amplicon size: | 189 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | IVS2-2 / | CTGGGAGGCCAAGGTATCTAAT | 22 bp | IVS 2 ADH1 |
| Primer reverse | PAT-B / | GCTGCTGTAGCTGGCCTAATCT | 22 bp | pat |
