QL-CON-00-002
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Tomato Nema 282F | (Tomato)
|
| Genetic target: | T-nos / PG |
| Description: | Qualitative PCR method for detection of the junction between the polygalacturonase gene (PG) and the nopaline synthase (nos) terminator (ISO 21569:2005) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex) |
| Amplicon sequence: |
GGATCCTTAGAAGCATCTAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNCTGTTGCCGGTCTTGCGATG |
| Amplicon size: | 350 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | PG34L / | GGATCCTTAGAAGCATCTAGT | 21 bp | PG |
| Primer reverse | t-Nos / | CATCGCAAGACCGGCAACAG | 20 bp | T-nos |
