ID   QT-TAX-ZM-007; SV 0; linear; genomic DNA; STS; PLN; 79 BP.
XX
AC   ;
XX
DT   21-DEC-2022
DT   21-DEC-2022
XX
DE   Quantitative droplet digital PCR method for detection of maize high-mobility-group gene (Gatto et al., 2022).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-79
RA   Gatto F., Savini C., Sacco MG., Vinciguerra D., Buttinger G., Corbisier P., Mazzara M., Emons H.;
RT   "Single and multi-laboratory validation of a droplet digital PCR method";
RL   Food Control, 140:109117 (2022).
RX   DOI=10.1016/j.foodcont.2022.109117
XX
RN   [2]
RP   1-79
RT   "See Cross-references below";
RL   Online Publication (2022).
XX
DR   GMOMETHODS; QT-EVE-ZM-032;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..79
FT                   /standard_name="PCR 79 bp amplicon"
FT                   /note="taxon-specific RT-PCR for maize"
FT                   /target="high-mobility-group (hmg) gene"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: ZM1-F"
FT                   /note="TTGGACTAGAAATCTCGTGCTGA"
FT                   /target="hmg"
FT   primer_bind     complement(34..56)
FT                   /standard_name="RT-PCR probe: Probe ZM1"
FT                   /note="HEX-CAATCCACACAAACGCACGCGTA-TAMRA"
FT   primer_bind     complement(58..79)
FT                   /standard_name="Primer reverse: ZM1-R"
FT                   /note="GCTACATAGGGAGCCTTGTCCT"
FT                   /target="hmg"
XX
SQ   Sequence 79 BP; 16 A; 13 C; 22 G; 28 T; 0 other;
     ttggactaga aatctcgtgc tgannnnnnn nnntacgcgt gcgtttgtgt ggattgnagg        60
     acaaggctcc ctatgtagc                                                     79
//