ID   QT-CON-00-008; SV 0; linear; genomic DNA; STS; SYN; 133 BP.
XX
AC   MON-00021-9;
XX
DT   15-SEP-2009
DT   05-OCT-2016
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DE   Quantitative PCR method for detection of the junction between an optimized transit peptide sequence and the point mutated epsps gene from maize.
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KW   construct_specific.
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OS   Zea mays (maize) - event GA21 (MON-00021-9)
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RN   [1]
RP   1-133
RA   Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J.,
RA   Akiyama H., Goda Y., Toyoda M., Hino A.;
RT   "Validation of real-time PCR analyses for line-specific quantitation of
RT   genetically modified maize and soybean using new reference molecules";
RL   J AOAC Int 85:1119-1126 (2002).
RX   PUBMED; 12374412.
XX
RN   [2]
RP   1-133
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [3]
RP   1-133
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-CON-00-008.pdf
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DR   GMOMETHODS; QT-TAX-ZM-006;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..133
FT                   /standard_name="PCR 133 bp amplicon"
FT                   /note="Construct-specific RT-PCR"
FT                   /target="Junction region between an optimized transit peptide sequence (OTP) and point mutated epsps gene (mEPSPS) from maize"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: GA21 3-5'"
FT                   /note="GAAGCCTCGGCAACGTCA"
FT                   /target="OTP"
FT   primer_bind     29..48
FT                   /standard_name="RT-PCR probe: GA21-2-Taq"
FT                   /note="FAM-AAGGATCCGGTGCATGGCCG-TAMRA"
FT   primer_bind     complement(114..133)
FT                   /standard_name="Primer reverse: GA21 3-3'"
FT                   /note="ATCCGGTTGGAAAGCGACTT"
FT                   /target="mEPSPS"
XX
SQ   Sequence 133 BP; 28 A; 42 C; 44 G; 19 T; 0 other;
     gaagcctcgg caacgtcann nnnnnnnnaa ggatccggtg catggccgnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagtcgc       120
     tttccaaccg gat                                                          133
//