ID   QT-CON-00-004; SV 0; linear; genomic DNA; STS; SYN; 113 BP.
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AC   MON-00810-6;
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DT   15-SEP-2009
DT   05-OCT-2016
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DE   Quantitative PCR method for detection of the junction between the intron 1 from the maize hsp70 gene and a
DE   synthetic cry1A(b) gene.
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KW   construct_specific.
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OS   Zea mays (maize) - event MON810 (MON-00810-6)
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RN   [1]
RP   1-113
RA   Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J.,
RA   Akiyama H., Goda Y., Toyoda M., Hino A.;
RT   "Validation of real-time PCR analyses for line-specific quantitation of
RT   genetically modified maize and soybean using new reference molecules";
RL   J AOAC Int 85:1119-1126 (2002).
RX   PUBMED; 12374412.
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RN   [2]
RP   1-113
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
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RN   [3]
RP   1-113
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-CON-00-004.pdf
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DR   GMOMETHODS; QT-TAX-ZM-006;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..113
FT                   /standard_name="PCR 113 bp amplicon"
FT                   /note="Construct-specific RT-PCR"
FT                   /target="Junction region between the Intron 1 from the maize hsp70 gene (IVS-HSP) and a synthetic cry1A(b) gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: M810 2-5'"
FT                   /note="GATGCCTTCTCCCTAGTGTTGA"
FT                   /target="IVS 1 hsp70"
FT   primer_bind     65..90
FT                   /standard_name="RT-PCR probe: M810-Taq"
FT                   /note="FAM-AGATACCAAGCGGCCATGGACAACAA-TAMRA"
FT   primer_bind     complement(93..113)
FT                   /standard_name="Primer reverse: M810 2-3'"
FT                   /note="GGATGCACTCGTTGATGTTTG"
FT                   /target="cry1A(b)"
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SQ   Sequence 113 BP; 31 A; 32 C; 20 G; 30 T; 0 other;
     gatgccttct ccctagtgtt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnagatac caagcggcca tggacaacaa nncaaacatc aacgagtgca tcc              113
//