ID   QL-CON-00-009; SV 0; linear; genomic DNA; STS; SYN; 83 BP.
XX
AC   ;
XX
DT   04-AUG-2009
DT   06-OCT-2016
XX
DE   Qualitative PCR method for detection of the junction between cry1A(b)/cry1A(c) and DNA spacer sequences in the context of Commission Decision 2008/289/EC)
XX
KW   construct_specific, EU-RL_GMFF_in-house_verified.
XX
OS   Oryza sativa (rice) - event Bt63
XX
RN   [1]
RP   1-83
RA   Savini C., Querci M., Ermolli M., Mazzara M., Cordeil S., Van den Eede
RA   G.;
RT   "Report on the Verification of the Performance of a Method for the
RT   Detection of 'Bt63' Rice Using Real-Time PCR";
RL   Online Publication (2008).
RX   CRL=Bt63_Rice_verification_report_final.pdf
XX
RN   [2]
RP   1-83
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-CON-00-009.pdf
XX
DR   GMOMETHODS; QL-TAX-OS-003;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..83
FT                   /standard_name="PCR 83 bp amplicon"
FT                   /note="construct-specific RT-PCR"
FT                   /target="Junction region between the cry1A(b)/cry1A(c) fusion gene and the DNA spacer sequences linking the fusion gene to the nopaline synthase terminator (T-nos)"
FT   primer_bind     1..25
FT                   /standard_name="Primer forward: T51F"
FT                   /note="GACTGCTGGAGTGATTATCGACAGA"
FT                   /target="cry1Ac"
FT   primer_bind     27..58
FT                   /standard_name="RT-PCR probe: T51p"
FT                   /note="FAM-TCGAGTTCATTCCAGTTACTGCAACACTCGAG-TAMRA"
FT   primer_bind     complement(60..83)
FT                   /standard_name="Primer reverse: T51R"
FT                   /note="AGCTCGGTACCTCGACTTATTCAG"
FT                   /target="DNA spacer sequences"
XX
SQ   Sequence 83 BP; 22 A; 18 C; 22 G; 21 T; 0 other;
     gactgctgga gtgattatcg acagantcga gttcattcca gttactgcaa cactcgagnc        60
     tgaataagtc gaggtaccga gct                                                83
//