ID   QT-CON-00-001; SV 0; linear; genomic DNA; STS; SYN; 121 BP.
XX
AC   MON-04032-6;
XX
DT   15-SEP-2009
DT   05-OCT-2016
XX
DE   Quantitative PCR method for detection of the junction between the chloroplast transit peptide and the CP4 epsps gene.
XX
KW   construct_specific.
XX
OS   Glycine max (soybean) - event GTS-40-3-2 (MON-04032-6)
XX
RN   [1]
RP   1-121
RA   Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J.,
RA   Akiyama H., Goda Y., Toyoda M., Hino A.;
RT   "Validation of real-time PCR analyses for line-specific quantitation of
RT   genetically modified maize and soybean using new reference molecules";
RL   J AOAC Int 85:1119-1126 (2002).
RX   PUBMED; 12374412.
XX
RN   [2]
RP   1-121
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [3]
RP   1-121
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-CON-00-001.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-007;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..121
FT                   /standard_name="PCR 121 bp amplicon"
FT                   /note="Construct-specific RT-PCR"
FT                   /target="Junction region between the chloroplast transit peptide (CTP) sequence from Petunia hybrida epsps gene and the CP4 epsps gene from Agrobacterium tumefasciens"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: RRS 01-5'"
FT                   /note="CCTTTAGGATTTCAGCATCAGTGG"
FT                   /target="CTP"
FT   primer_bind     62..80
FT                   /standard_name="RT-PCR probe: RRS-Taq"
FT                   /note="FAM-CGCAACCGCCCGCAAATCC-TAMRA"
FT   primer_bind     complement(104..121)
FT                   /standard_name="Primer reverse: RRS 01-3'"
FT                   /note="GACTTGTCGCCGGGAATG"
FT                   /target="CP4-EPSPS"
XX
SQ   Sequence 121 BP; 23 A; 45 C; 29 G; 24 T; 0 other;
     cctttaggat ttcagcatca gtggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     ncgcaaccgc ccgcaaatcc nnnnnnnnnn nnnnnnnnnn nnncattccc ggcgacaagt       120
     c                                                                       121
//