ID   QL-TAX-SL-001; SV 0; linear; genomic DNA; STS; PLN; 384 BP.
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AC   ;
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DT   05-MAY-2009
DT   20-SEP-2010
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DE   Qualitative PCR method for detection of tomato polygalacturonase gene
DE   (ISO/FDIS 21569:2005).
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KW   taxon_specific, validated_independently.
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OS   Solanum lycopersicum (tomato)
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RN   [1]
RP   1-384
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Qualitative nucleic acid based
RT   methods";
RL   ISO 21569:1-69 (2005).
RX   ISO=34614
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RN   [2]
RP   1-384
RT   "Screening procedure for the detection of genetically modified DNA
RT   sequences in foods by identification of DNA sequences that frequently
RT   occur in genetically modified organisms. No. L 00.00-31";
RL   Online Publication (1998).
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RN   [3]
RP   1-384
RT   "Detection of a genetic modification of tomatoes by amplification of the
RT   modified DNA sequence by means of the polymerase chain reaction (PCR) and
RT   hybridization of the PCR product with a DNA probe or restriction analysis
RT   of the PCR product, No. L25.03.01";
RL   Online Publication (1999).
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RN   [4]
RP   1-384
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-TAX-SL-001.pdf
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DR   GMOMETHODS; QL-CON-00-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..384
FT                   /standard_name="PCR 384 bp amplicon"
FT                   /note="Taxon-specific PCR for tomato"
FT                   /target="polygalacturonase (PG) gene"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: PG34L"
FT                   /note="GGATCCTTAGAAGCATCTAGT"
FT                   /target="PG"
FT   primer_bind     complement(364..384)
FT                   /standard_name="Primer reverse: PG34R"
FT                   /note="CGTTGGTGCATCCCTGCATGG"
FT                   /target="PG"
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SQ   Sequence 384 BP; 116 A; 60 C; 73 G; 135 T; 0 other;
     ggatccttag aagcatctag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       180
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       240
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       300
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       360
     nnnccatgca gggatgcacc aacg                                              384
//