ID   QL-TAX-PS-001; SV 1; linear; genomic DNA; STD; PLN; 78 BP.
XX
AC   ;
XX
DT   30-JUL-2008
DT   22-DEC-2016
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DE   Qualitative PCR method for detection of pea lectin gene (Debode et al., 2016).
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KW   taxon_specific, validated_independently.
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OS   Pisum sativum (pea)
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RN   [1]
RP   1-78
RA   Debode F., Huber I., Macarthur R., Rischitor P.E., Mazzara M., Herau V., Sebah D., Dobnik D., Broeders S., Roosens N.H., Busch U., Berben G., Morisset D., Zel J.;
RT   " Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection";
RL   Food Control 73:452-461 (2017).
RX   DOI=10.1016/j.foodcont.2016.08.037.
XX
RN   [2]
RP   1-78
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QL-TAX-PS-001.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..78
FT                   /standard_name="PCR 78 bp amplicon"
FT                   /note="taxon-specific RT-PCR for pea"
FT                   /target="pea lectin (psl) gene"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: lecPea1-F"
FT                   /note="TGGAATCGATGTGAACAGTATCAA"
FT                   /target="psl"
FT   primer_bind     26..52
FT                   /standard_name="RT-PCR probe: lecPea1-P"
FT                   /note="FAM-TCCGTAAACACTAAGTCGTGGAAGTTG-TAMRA"
FT   primer_bind     complement(54..78)
FT                   /standard_name="Primer reverse: lecPea1-R"
FT                   /note="ACAACATTAGCCTCTTCACCATTCT"
FT                   /target="psl"
XX
SQ   Sequence 78 BP; 26 A; 10 C; 22 G; 20 T; 0 other;
     tggaatcgat gtgaacagta tcaaatccgt aaacactaag tcgtggaagt tgcagaatgg        60
     tgaagaggct aatgttgt                                                      78
//