ID   QL-TAX-OS-006; SV 0; linear; genomic DNA; STS; PLN; 277 BP.
XX
AC   ;
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DT   23-JUN-2009
DT   01-OCT-2010
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DE   Qualitative PCR method for detection of rice sucrose-phosphate synthase gene
DE   (Jiang et al., 2009).
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KW   taxon_specific, validated_independently.
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OS   Oryza sativa (rice)
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RN   [1]
RP   1-277
RA   Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang
RA   D.;
RT   "International collaborative study of the endogenous reference gene,
RT   sucrose phosphate synthase (SPS), used for qualitative and quantitative
RT   analysis of genetically modified rice";
RL   J. Agric. Food Chem. 57:3525-3532 (2009).
RX   PUBMED; 19326953.
RX   DOI=10.1021/jf803166p
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RN   [2]
RP   1-277
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-TAX-OS-006.pdf
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FH   Key             Location/Qualifiers
FH
FT   STS             1..277
FT                   /standard_name="PCR 277 bp amplicon"
FT                   /note="Taxon-specific PCR for rice"
FT                   /target="sucrose-phosphate synthase (SPS) gene"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: SPS-1F"
FT                   /note="TTGCGCCTGAACGGATAT"
FT                   /target="SPS"
FT   primer_bind     complement(259..277)
FT                   /standard_name="Primer reverse: SPS-2R"
FT                   /note="GGAGAAGCACTGGACGAGG"
FT                   /target="SPS"
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SQ   Sequence 277 BP; 57 A; 113 C; 60 G; 47 T; 0 other;
     ttgcgcctga acggatatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       180
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       240
     nnnnnnnnnn nnnnnnnncc tcgtccagtg cttctcc                                277
//