ID   QL-TAX-OS-002; SV 0; linear; mRNA; STS; PLN; 68 BP.
XX
AC   ;
XX
DT   09-JUN-2008
DT   25-NOV-2021
XX
DE   Qualitative PCR method for detection of rice Phospholipase D alpha 2 gene.
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Oryza sativa (rice)
XX
RN   [1]
RP   1-68
RA   Mazzara M., Cordeil S., Van Den Eede G.;
RT   "Report on the Verification of an Event-specific Detection Method for Identification of Rice GM-event LLRICE601 Using a Real-time PCR Assay";
RL   Online Publication (2006).
RX   EURL_GMFF=Verification Report LLRice601 event.pdf
XX
RN   [2]
RP   1-68
RA   Bayer CropScience;
RT   "Grain testing method for detection of rice GM event LLRICE601 using RT-PCR protocols PGS0505 and PGS0476";
RL   Online Publication (2006).
RX   CRL-DOC=P35S-bar sqRT-PCR - PGS0494-476 310806.pdf
XX
RN   [3]
RP   1-68
RA   US Department of Agriculture;
RT   "Verification of the Bayer CropScience method for the detection of LL601 in rice using real-time PCR";
RL   Online Publication (2006).
RX   CRL-DOC=35SBarRiceVerficationReportrev.pdf
XX
RN   [4]
RP   1-68
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Report on the Verification of a Construct-specific Detection Method for
RT   Identification of Rice GM-Events containing P35S::BAR using a Real-time
RT   PCR Assay";
RL   Online Publication (2006).
RX   CRL-DOC=Verification Report 35S-BAR CRL.pdf
XX
RN   [5]
RP   1-68
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QL-EVE-OS-001;
DR   GMOMETHODS; QL-CON-00-015;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..68
FT                   /standard_name="PCR 68 bp amplicon"
FT                   /note="taxon-specific RT-PCR"
FT                   /target="phospholipase D (PLD) gene"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: KVM159"
FT                   /note="TGGTGAGCGTTTTGCAGTCT"
FT                   /target="PLD"
FT   primer_bind     22..44
FT                   /standard_name="RT-PCR probe: TM013"
FT                   /note="VIC-TGTTGTGCTGCCAATGTGGCCTG-TAMRA"
FT   primer_bind     complement(47..68)
FT                   /standard_name="Primer reverse: KVM160"
FT                   /note="CTGATCCACTAGCAGGAGGTCC"
FT                   /target="PLD"
XX
SQ   Sequence 68 BP; 8 A; 14 C; 22 G; 21 T; 3 other;
     tggtgagcgt tttgcagtct ntgttgtgct gccaatgtgg cctgnnggac ctcctgctag        60
     tggatcag                                                                 68
//