ID QT-EVE-BN-005; SV 0; linear; genomic DNA; STS; SYN; 187 BP. XX AC ACS-BN004-7; XX DT 22-NOV-2011 DT 16-JAN-2008 XX DE Quantitative PCR method for detection of oilseed rape event Ms1 (verified by the EU-RL GMFF in the context of Commission Decision 2007/305/EC) XX KW event_specific, EU-RL_GMFF_in-house_verified. XX OS Brassica napus (oilseed rape) - event Ms1 (ACS-BN004-7) XX RN [1] RP 1-187 RA Savini C., ; RT "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape MS1 Using Real-time PCR - Validation Report and Protocol"; RL Online Publication (2011). RX EURL_GMFF=CRLVL1104 VR.pdf RX EURL_GMFF=CRLVL1104 VP.pdf XX RN [2] RP 1-187 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2011). RX PCR=QT-EVE-BN-005.pdf XX DR GMOMETHODS; QT-TAX-BN-012; XX FH Key Location/Qualifiers FH FT STS 1..187 FT /standard_name="PCR 187 bp amplicon" FT /note="event-specific RT-PCR" FT /target="3'integration border region (IBR) between the insert of oilseed rape event Ms1 and the oilseed rape host genome"; FT primer_bind 1..20 FT /standard_name="Primer forward: MLD025" FT /note="ACGCTGCGGACATCTACATT" FT /target="insert" FT primer_bind 79..105 FT /standard_name="RT-PCR probe: TM030" FT /note="FAM-CTCATTGCTGATCCACCTAGCCGACTT-TAMRA" FT primer_bind complement(166..187) FT /standard_name="Primer reverse: MDB175" FT /note="CTAGATCGGAAGCTGAAGATGG" FT /target="3'-host genome" XX SQ Sequence 187 BP; 53 A; 50 C; 21 G; 63 T; 0 other; acgctgcgga catctacatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnct cattgctgat ccacctagcc gacttnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatc ttcagcttcc 180 gatctag 187 //