ID   QT-EVE-BN-008; SV 0; linear; genomic DNA; STS; SYN; 95 BP.
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AC   ACS-BN007-1;
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DT   29-NOV-2011
DT   11-FEB-2008
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DE   Quantitative PCR method for detection of oilseed rape event Topas 19/2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/307/EC)
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KW   event_specific, EU-RL_GMFF_in-house_verified.
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OS   Brassica napus (oilseed rape) - event Topas 19/2 (ACS-BN007-1)
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RN   [1]
RP   1-95
RA   Mazzara M.,;
RT   "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape Topas 19/2 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=CRLVL1204 VR.pdf
RX   EURL_GMFF=CRLVL1204 VP.pdf
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RN   [2]
RP   1-95
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2011).
RX   PCR=QT-EVE-BN-008.pdf
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DR   GMOMETHODS; QT-TAX-BN-012;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..95
FT                   /standard_name="PCR 95 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="3'integration border region (IBR) between the insert of oilseed rape event Topas 19/2 and the oilseed rape host genome";
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: MDB685"
FT                   /note="GTTGCGGTTCTGTCAGTTCC"
FT                   /target="insert";
FT   primer_bind     38..54
FT                   /standard_name="RT-PCR probe:  TM029"
FT                   /note="FAM-TCCCGCGTCATCGGCGG-TAMRA"
FT   primer_bind     complement(76..95)
FT                   /standard_name="Primer reverse: KVM180"
FT                   /note="CGACCGGCGCTGATATATGA"
FT                   /target="3'-host genome"
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SQ   Sequence 95 BP; 16 A; 27 C; 29 G; 23 T; 0 other;
     gttgcggttc tgtcagttcc nnnnnnnnnn nnnnnnntcc cgcgtcatcg gcggnnnnnn        60
     nnnnnnnnnn nnnnntcata tatcagcgcc ggtcg                                   95
//