ID   QL-TAX-GM-001; SV 1; linear; genomic DNA; STD; PLN; 81 BP.
XX
AC   ;
XX
DT   27-APR-1993
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Glycine max (soybean) 
XX
RN   [1]
RP   1-81
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (2014).
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-81
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-TAX-GM-001.pdf
XX
RN   [3]
RP   1-81
RT   "See Cross-references below";
RL   Online Publication (2014).
XX
DR   GMOMETHODS; QL-ELE-00-017;
DR   GMOMETHODS; QL-ELE-00-018;
DR   GMOMETHODS; QL-ELE-00-019;
DR   GMOMETHODS; QL-ELE-00-020;
DR   GMOMETHODS; QL-ELE-00-021;
DR   GMOMETHODS; QL-ELE-00-022;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..81
FT                   /standard_name="PCR 81 bp amplicon"
FT                   /note="plant-specific PCR"
FT                   /target="lectin (Le1) gene"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: SLTM 1"
FT                   /note="AACCGGTAGCGTTGCCAG"
FT                   /target="Le1"
FT   primer_bind     complement(63..81)
FT                   /standard_name="Primer reverse: SLTM 2"
FT                   /note="AGCCCATCTGCAAGCCTTT"
FT                   /target="Le1"
XX
SQ   Sequence 81 BP; 17 A; 27 C; 17 G; 20 T; 0 other;
     aaccggtagc gttgccagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnaaaggctt gcagatgggc t                                                  81
//