ID QL-TAX-DC-001; SV 0; linear; genomic DNA; STS; PLN; 1279 BP. XX AC ; XX DT 20-SEP-2012 DT 15-DEC-2017 XX DE Qualitative PCR method for detection of carnation anthocyanidin synthase gene. XX KW taxon_specific, validated_in_combination. XX OS Dianthus caryophyllus (carnation) XX RN [1] RP 1-1279 RA Savini C., Querci M., Moens W., Mazzara M., Cordeil S., Van den Eede G.; RT "Report on the Testing of a PCR-based Detection Method for Identification RT of FlorigeneTM Moonlite GM Carnation - Protocol Version 2"; RL Online Publication (2007). RX EURL_2001=CRL_Report_Flor_Moonlite_v2.pdf XX RN [2] RP 1-1279 RA Savini C., Brustio R., Bogni A., Mazzara M., Kreysa J.; RT "Report on the single-laboratory validation of a PCR-based Detection Method for Identification of FlorigeneTM IFD-25958-3 GM Carnation - Validation Report and Validated Method"; RL Online Publication (2012). RX DOI=10.2788/76760 XX RN [3] RP 1-1279 RA Savini C., Pinski G., Mazzara M., Kreysa J.; RT "Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of FlorigeneTM 26407 GM Carnation - Validation Report and Validated Method"; RL Online Publication (2013). RX DOI=10.2788/34771 XX RN [4] RP 1-1279 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of SHD-27531-4 GM Carnation - JRC Validated Methods, Reference Methods and Measurement Reports"; RL Online Publication (2016). RX EURL_2001=EURL-EV-01-13-Final-Report.pdf XX RN [5] RP 1-1279 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of GM-line FLO-40685-2 Carnation - JRC Validated Methods, Reference Methods and Measurement Reports"; RL Online Publication (2016). RX EURL_2001=EURL-EV-02-13-VR.pdf XX RN [6] RP 1-1279 RT "See Cross-references below"; RL Online Publication (2016). XX DR GMOMETHODS; QL-EVE-DC-001; DR GMOMETHODS; QL-EVE-DC-003; DR GMOMETHODS; QL-EVE-DC-004; DR GMOMETHODS; QL-EVE-DC-005; DR GMOMETHODS; QL-EVE-DC-006; XX FH Key Location/Qualifiers FH FT /note="This sequence has been obtained by manual FT annotation of record GenBank:AB727362.2, Dianthus FT caryophyllus genomic DNA, anthocyanidin synthase promoter FT region. " FT STS 1..1279 FT /standard_name="PCR 1279 bp amplicon" FT /note="taxon-specific PCR for carnation"; FT /target="anthocyanidin synthase gene" FT primer_bind 1..22 FT /standard_name="Primer forward: ANS.F" FT /note="CTAGATCGGAGGTCACCATACC" FT /target="ANS" FT primer_bind complement(1259..1279) FT /standard_name="Primer reverse: ANS.R" FT /note="GAAACCGTGACCATGGTCTCG" FT /target="ANS" XX SQ Sequence 1279 BP; 352 A; 250 C; 272 G; 405 T; 0 other; ctagatcgga ggtcaccata cccgtgaaga tttttctgtg agaggaaaag aacccaagga 60 cgaggttcaa atctacacat ggaaagacgc cacgcttcgt gagttaactg accttgtatg 120 tcgccatcgc ttagcgtagc gctgaacatc gttttcaccc tgctccatcc atcaaccatt 180 tattggtctt atacatgtgt gattgcgttg ttcttacatt taggtgaaag acgtttctcc 240 agctgctagg agtcgagatg cgaaattgtc gtttgcgact gtataccttg atagaaatgg 300 atgcatgcaa gtaaagaagg tatcttctaa ttcatctttc gtagagacat agcgtgaatt 360 tggacggggt ctttggtttg agaaagataa cagctttacg tatttttgta gatgggtgaa 420 accttttcaa atccgtataa gcgtaaagac gacaactggg ctttagggga cacattcttt 480 caggtataat tgatgcgact aacaatagtc tccactgatc atattctact cttctacgtt 540 cgatactgac tgtttctggt tatttggtag acaggagatt atttggacgt agcaattcag 600 tagcgtagag atgtttccac acgtgttatc gtaaaagaag caagataagc ctaatgccta 660 gggtggtggt atgacttccg ttgcttatcg atcgtgcttg taagtaattt ccgtcttatc 720 ttttcctgtt atataaagtt aatcttctct aggactttca tgaaccttgt ttgtgtattt 780 atttctcgat caacatgata gagctagttt ttaagcaacg tatactagta gtctattgga 840 agttaagaca cggttcttaa aaaggtacga tccaagtgaa gcatgttaga tatgacactt 900 tcttctaggg acgactctcg tatgccaccc gactttttca attttttttg tgaatgttag 960 atgtgtgtat ataatgcatc cgaaagatgt ctcaacgaac aaatgagcca cctacttcga 1020 tcactcgcta tcaatgttat taatgccttg ttgattttaa tagttgatca ataatagtaa 1080 aatctattca agggtatagt ctcccgttca cactcatcgg ggttacacta gcgagctcca 1140 ttaatcggtg ccttaatcga gacgctaaga actataccat gacctagtca gcgccatggg 1200 actgatgtag gccacacaat ctcgatgatc cgaaaacgct agagttcaag acctagttcg 1260 agaccatggt cacggtttc 1279 //
