ID QT-EVE-GM-014; SV 0; linear; genomic DNA; STS; SYN; 105 BP. XX AC DAS-81419-2; XX DT 19-JUN-2013 DT 06-MAY-2015 XX DE Quantitative PCR method for detection of soybean event DAS-81419-2 (EURL GMFF, 2015). XX KW event_specific. XX OS Glycine max (soybean)- event DAS-81419-2(DAS-81419-2) XX RN [1] RP 1-105 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Soybean DAS-81419-2 by Real-time PCR - Validation Report and Validated Method"; RL Online Publication (2015). RX EURL_GMFF=EURLVL0313VR%20Soybean%20DAS-81419-2%20.pdf RX EURL_GMFF=EURLVL0313VP_Validated%20method.pdf XX RN [2] RP 1-105 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2015). RX PCR=QT-EVE-GM-014.pdf XX DR GMOMETHODS; QT-TAX-GM-002; XX FH Key Location/Qualifiers FH FT STS 1..105 FT /standard_name="PCR 105 bp amplicon" FT /note="Event-specific RT-PCR"; FT /target="5' integration border region (IBR) between the insert of soybean event DAS-81419-2 and the soybean host genome"; FT primer_bind 1..29 FT /standard_name="Primer forward: DAS81419-f2" FT /note="TCTAGCTATATTTAGCACTTGATATTCAT" FT /target="5'-host genome" FT primer_bind complement(57..80) FT /standard_name="RT-PCR probe: DAS81419-p3" FT /note="FAM-ATCAACAGGCACCGATGCGCACCG-TAMRA" FT primer_bind complement(85..105) FT /standard_name="Primer reverse: DAS81419-r1" FT /note="GCTTCAAGATCCCAACTTGCG" FT /target="insert" XX SQ Sequence 105 BP; 26 A; 24 C; 23 G; 32 T; 0 other; tctagctata tttagcactt gatattcatn nnnnnnnnnn nnnnnnnnnn nnnnnncggt 60 gcgcatcggt gcctgttgat nnnncgcaag ttgggatctt gaagc 105 //