ID   QL-TAX-BN-003; SV 1; linear; genomic DNA; STD; PLN; 85 BP.
XX
AC   ;
XX
DT   18-MAY-1989
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of oilseed rape cruciferin A gene (Barbau-Piednoir et al., 2014).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Brassica napus (oilseed rape)
XX
RN   [1]
RP   1-85
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (2014).
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-85
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-TAX-BN-003.pdf
XX
RN   [3]
RP   1-85
RT   "See Cross-references below";
RL   Online Publication (2014).
XX
DR   GMOMETHODS; QL-ELE-00-017;
DR   GMOMETHODS; QL-ELE-00-018;
DR   GMOMETHODS; QL-ELE-00-019;
DR   GMOMETHODS; QL-ELE-00-020;
DR   GMOMETHODS; QL-ELE-00-021;
DR   GMOMETHODS; QL-ELE-00-022;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..85
FT                   /standard_name="PCR 85 bp amplicon"
FT                   /note="taxon-specific PCR for oilseed rape"
FT                   /target="cruciferin A (CruA) gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: Cru770F"
FT                   /note="CAGCTCAACAGTTTCCAAACGA"
FT                   /target="CruA"
FT   primer_bind     complement(66..85)
FT                   /standard_name="Primer reverse: Cru770 R"
FT                   /note="CGACCAGCCTCAGCCTTAAG"
FT                   /target="CruA"
XX
SQ   Sequence 85 BP; 22 A; 24 C; 22 G; 17 T; 0 other;
     cagctcaaca gtttccaaac gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnncttaa ggctgaggct ggtcg                                              85
//