ID   QL-TAX-AT-001; SV 1; circular; genomic DNA; STD; BCT; 86 BP.
XX
AC   ;
XX
DT   11-NOV-2023
DT   11-NOV-2023
XX
DE   Qualitative PCR method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022).
XX
KW   taxon_specific, validated_independently.
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OS   Agrobacterium tumefaciens
XX
RN   [1]
RP   1-86
RT   "Horizontal methods for molecular biomarker analysis - Methods of
RT   analysis for the detection of genetically modified organisms and derived
RT   products - Part 7: Real-time PCR based methods for the detection of CaMV
RT   and Agrobacterium Ti-plasmid derived DNA sequences";
RL   ISO/TS 21569-7(2022).
RX   ISO=84434
XX
RN   [2]
RP   1-86
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2023).
RX   PCR=QL-TAX-AT-001.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..86
FT                   /standard_name="PCR 86 bp amplicon"
FT                   /note="Taxon-specific RT-PCR"
FT                   /target="nopaline synthase (nos ) gene from Agrobacterium
FT                   tumefaciens"
FT   primer_bind     1..19
FT                   /standard_name="Primer reverse: At-nop-r2"
FT                   /note="TGCGAGTTCRCCGTTGAAG"
FT                   /target="nos"
FT   primer_bind     complement(41..61)
FT                   /standard_name="RT-PCR probe: At-nop-s1"
FT                   /note="FAM-CCGTGCGGACGTTCACGACAG-BHQ1"
FT   primer_bind     complement(62..86)
FT                   /standard_name="Primer forward: At-nop-f2"
FT                   /note="CCAGCCRTSTACTGATTATTGTMAC"
FT                   /target="nos"
XX
SQ   Sequence 86 BP; 18 A; 19 C; 28 G; 21 T; 0 other;
     tgcgagttcr ccgttgaagt tggcgagttc gttgacgaag ctgtcgtgaa cgtccgcacg        60
     ggtkacaata atcagtasay ggctgg                                            86
//