ID   QT-EVE-GM-009; SV 0; linear; genomic DNA; STS; SYN; 99 BP.
XX
AC   DP-356043-5;
XX
DT   23-MAY-2007
DT   15-MAR-2012
XX
DE   Quantitative PCR method for detection of soybean event DP-356043-5
DE   (Mazzara et al., 2009).
XX
KW   event_specific.
XX
OS   Glycine max (soybean) - event DP-356043-5 (DP-356043-5)
XX
RN   [1]
RP   1-99
RA   Mazzara M., Munaro B., Grazioli E., Savini C., Charles Delobel C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Soybean Event DP-356043-5 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2009).
RX   DOI=10.2788/5751
XX
RN   [2]
RP   1-99
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-GM-009.pdf
XX
DR   GMOMETHODS; QT-TAX-GM-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..99
FT                   /standard_name="PCR 99 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of soybean event DP-356043-5 and the soybean host genome"
FT   primer_bind     1..26
FT                   /standard_name="Primer forward: DP356-f1"
FT                   /note="GTCGAATAGGCTAGGTTTACGAAAAA"
FT                   /target="5'-host genome"
FT   primer_bind     40..69
FT                   /standard_name="RT-PCR probe: DP356-p"
FT                   /note="FAM-CTCTAGAGATCCGTCAACATGGTGGAGCAC-TAMRA"
FT   primer_bind     complement(71..99)
FT                   /standard_name="Primer reverse: DP356-r1"
FT                   /note="TTTGATATTCTTGGAGTAGACGAGAGTGT"
FT                   /target="insert"
XX
SQ   Sequence 99 BP; 29 A; 20 C; 17 G; 19 T; 14 other;
     gtcgaatagg ctaggtttac gaaaaannnn nnnnnnnnnc tctagagatc cgtcaacatg        60
     gtggagcacn acactctcgt ctactccaag aatatcaaa                               99
//