ID   QL-EVE-DC-004; SV 0; linear; genomic DNA; STS; SYN; 491 BP.
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AC   IFD-26407-2;
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DT   17-SEP-2012
DT   18-MAY-2016
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DE   Qualitative PCR method for detection of carnation event 26407 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/694).
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KW   event_specific, EU-RL_GMFF_in-house_verified.
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OS   Dianthus caryophyllus (carnation) - event 26407 (IFD-26407-2)
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RN   [1]
RP   1-491
RA   Savini C., Pinski G., Mazzara M., Kreysa J.;
RT   "Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of FlorigeneTM 26407 GM Carnation - Validation Report and Validated Method";
RL   Online Publication (2013).
RX   DOI=10.2788/34771
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RN   [2]
RP   1-491
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QL-EVE-DC-004.pdf
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DR   GMOMETHODS; QL-TAX-DC-001;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..491
FT                   /standard_name="PCR 491 bp amplicon"
FT                   /note="Event-specific PCR";
FT                   /target="3' integration border region (IBR) between the insert of carnation event 26407 and the carnation host genome"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: RB Forward"
FT                   /note="ATTTCCACCTTCACCTACGATGG"
FT                   /target="insert"
FT   primer_bind     complement(471..491)
FT                   /standard_name="Primer reverse: IFD-26407-2 Reverse"
FT                   /note="CACGGGTACAAATTGGATGAG"
FT                   /target="3'-host genome"
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SQ   Sequence 491 BP; 157 A; 84 C; 86 G; 164 T; 0 other;
     atttccacct tcacctacga tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       180
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       240
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       300
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       360
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       420
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcatccaat       480
     ttgtacccgt g                                                            491
//